[연구데이터]Extracellular vesicles derived from DFO preconditioned canine AT-MSCs reprogram macrophages into M2 phase

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[연구데이터]Extracellular vesicles derived from DFO preconditioned canine AT-MSCs reprogram macrophages into M2 phase

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학술지명(Journal)

PLOS ONE

ISSN_ISBN

1932-6203

학술지볼륨권호(Volume)

16(7)

SCI구분

SCIE

논문제목(Title)

[연구데이터]Extracellular vesicles derived from DFO preconditioned canine AT-MSCs reprogram macrophages into M2 phase

주저자명(FirstAuthor)

Su-Min Park, Woo-Jin Song, Hwa-Young Youn

공동저자명(Co-Author)

Ju-Hyun An

공동저자명(Co-Author)

Jeong-Hwa Lee

공동저자명(Co-Author)

Kyung-Bo Kim

공동저자명(Co-Author)

Hyung-Kyu Chae

공동저자명(Co-Author)

Ye-In Oh

초록(Abstract)

Background
Mesenchymal stem/stromal cells (MSCs) are effective therapeutic agents that ameliorate inflammation through paracrine effect; in this regard, extracellular vesicles (EVs) have been frequently studied. To improve the secretion of anti-inflammatory factors from MSCs, preconditioning
with hypoxia or hypoxia-mimetic agents has been attempted and the molecular changes in preconditioned MSC-derived EVs explored. In this study, we aimed to investigate the increase of hypoxia-inducible factor 1-alpha (HIF-1α)/cyclooxygenase-2 (COX-2) in deferoxamine (DFO)-preconditioned canine MSC (MSCDFO) and whether these molecular changes were reflected on EVs. Furthermore, we focused on MSCDFO derived EVs
(EVDFO) could affect macrophage polarization via the transfer function of EVs.
Results
In MSCDFO, accumulation of HIF-1α were increased and production of COX-2 were activated. Also, Inside of EVDFO were enriched with COX-2 protein. To evaluate the transferring effect of EVs to macrophage, the canine macrophage cell line, DH82, was treated with EVs after lipopolysaccharide (LPS) stimulation. Polarization changes of DH82 were evaluated with quantitative real-time PCR and immunofluorescence analyses. When LPS-inducedDH82 was treated with EVDFO, phosphorylation of signal transducer and transcription3 (p-
STAT3), which is one of key factor of inducing M2 phase, expression was increased in DH82. Furthermore, treated with EVDFO in LPS-induced DH82, the expression of M1 markers were reduced, otherwise, M2 surface markers were enhanced. Comparing with EVDFO and EVnon.
Conclusion
DFO preconditioning in MSCs activated the HIF-1α/COX-2 signaling pathway; Transferring COX-2 through EVDFO could effectively reprogram macrophage into M2 phase by promoting the phosphorylation of STAT3.

학술지출판일자(PublicationDate)

2021-07-26

DOI

https://doi.org/10.1371/journal.pone.0254657

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20200203 PSM cATMSC GAPDH COX2 CXCR4 TSG6_data.xls

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20200414 PSM DH82 M1 GAPDH iNOS_data.xls

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20200414 PSM DH82 M1 IL-1b IL-6_data.xls

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2021-12-23

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컬렉션: [송우진] 제주 지역 개에서 유래한 중간엽줄기세포의 TSG-6 발현양과 항염증 효과와의 상관관계에 대한 평가(2021-2022)

제출자: 송우진

공개일자: 2021-12-23

DOI: https://doi.org/10.22686/idr/1

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Version 1 10.22686/idr/1 2021-12-23

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송우진 ( 2021-12-23 ) [연구데이터]Extracellular vesicles derived from DFO preconditioned canine AT-MSCs reprogram macrophages into M2 phase
https://doi.org/10.22686/idr/1

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[연구데이터]Extracellular vesicles derived from DFO preconditioned canine AT-MSCs reprogram macrophages into M2 phase

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