Asian Pacific Journal of Tropical Biomedicine

2221-1691

2022; 12(3): 115-123

SCIE

[논문]Aqueous extract of freeze-dried Protaetia brevitarsis larvae promotes osteogenesis by activating β-catenin signaling

Jayasingha Arachchige Chathuranga Chanaka Jayasingha, Gi-Young Kim

자야싱하, 김기영

Kyoung Tae Lee, Yung Hyun Choi, Chang-Hee Kang, Mi-Hwa Lee

Objective: To investigate the effect of an aqueous extract of
Protaetia brevitarsis (AEPB) on osteogenesis using preosteoblast
MC3T3-E1 cells and zebrafish larvae.
Methods: Flow cytometric analysis was used to measure the
cytotoxicy. Alkaline phosphatase activity was detetmined using
p-nitrophenyl phosphate as a substrate. Calcium deposition was
detected using alizarin red staining along with osteogenic marker
expression in preosteoblast MC3T3E1 cells. In addition, vertebral
formation in zebrafish larvae was detected using calcein staining and
osteogenic gene expression.
Results: AEPB highly promoted the expression of osteogenic
markers including runt-related transcription factor 2, osterix, and
alkaline phosphatase, along with elevated levels of mineralization in
MC3T3-E1 cells. Moreover, AEPB accelerated vertebral formation
in zebrafish larvae accompanied by upregulated expression of
osteogenic genes. FH535, an inhibitor of Wnt/β-catenin, suppressed
AEPB-induced osteogenic gene expression and vertebral formation,
indicating that AEPB stimulates osteogenesis by activating the Wnt/
β-catenin signaling pathway.
Conclusions: AEPB stimulates osteoblast differentiation and bone
formation by activating β-catenin. Therefore, AEPB is a promising
material that induces osteogenesis, and is useful for the treatment of
bone resorption diseases.

2022.05.10.

doi: 10.4103/2221-1691.338920