Histology and Histopathology

0213-3911

SCIE

[논문]Sargassum horneri extract containing polyphenol alleviates DNCB-induced atopic dermatitis in NC/Nga mice through restoring skin barrier function

Suyama Prasansali Mihindukulasooriya, Youngheun Jee

수야마, 지영은, 김효진(공동저자)

Duong Thi Thuy Dinh, Kalahe Hewage Iresha Nadeeka Madushani Herath, Hyo Jin Kim, Eui-Jeong Han, Jinhee Cho, Mi-Ok Ko, You-Jin Jeon, Ginnae Ahn

Atopic dermatitis (AD) is a chronic
inflammatory skin disease characterized by skin barrier
dysfunction. Sargassum horneri (S. horneri) is a brown
alga that has been widely used in traditional medicine of
eastern Asian countries. Recent studies proved that a
brown alga S. horneri has anti-inflammatory activity. In
this study, we investigated the effect of S. horneri
ethanol extract (SHE) against AD in 2,4-dinitrobenzene
(DNCB) induced AD in NC/Nga mice. We observed that
SHE treatment decreased the epidermal thickness and
epidermal hyperplasia that had been worsened through
DNCB application. Moreover, SHE significantly
inhibited the proliferation of mast cells and decreased
the expression of IL-13 on CD4+ cells prompted by
elevated thymic stromal lymphopoietin (TSLP)
expression in DNCB-induced AD in mice. We also
demonstrated that SHE directly inhibited the expression
of keratinocyte-produced TSLP known to exacerbate
skin barrier impairment. Especially, the decrease of
filaggrin, an integral component of proper skin barrier
function through a function in aggregating keratin
filaments, observed in DNCB-induced AD mice was
significantly improved when treated with SHE. More
importantly, we proved that SHE was able to decrease
the serum levels of IgG1 and IgG2a, two crucial factors
of AD, indicating the protective effect of SHE. Taken
together, our findings suggest that SHE may protect
NC/Nga mice against DNCB-induced AD via promoting
skin barrier function

2022.05.30.

DOI: 10.14670/HH-18-473