Current Microbiology

2.6

0343-8651

81:13

SCIE

[논문]Granulicatella seriolae sp. nov., a Novel Facultative Anaerobe Isolated from Yellowtail Marine Fish

Myunglip Lee, Yun Ji Choi

Adeel Farooq, Joon Bum Jeong and Man-Young Jung

A bacterial strain, designated as S8T, was isolated from the gut contents of Seriola quinqueradiata from the coastal sea area
of Jeju Island, South Korea. The strain is a Gram-staining positive, non-motile, non-spore-forming, facultative anaerobic
coccus. Optimal growth was observed at 30 °C, pH 8.0–9.0, and 0–0.5% w/v NaCl, under anaerobic conditions. The predominant fatty acids were C18:1 ω9c, C16:0, C18:0, and C16:1 ω9c, while quinone was not detected. The genome was 2,224,566
bp long, with a GC content of 38.2%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain S8T
had 96.2% similarity with Granulicatella adiacens ATCC 49175T, its closest known species according to nomenclature.
The DNA–DNA hybridization (dDDH), average nucleotide identity, and average amino acid identity values between strain
S8T and G. adiacens ATCC 49175T were 25.7%, 85.5%, and 77.2%, respectively, all of which fall below the recommended
threshold for species diferentiation. Based on genomic, phenotypic, and phylogenetic evidence, we propose that strain S8T
should be a novel species within the genus Granulicatella, for with the name Granulicatella seriolae sp. nov. is proposed.
The type strain is S8T (KCTC 43438T=JCM 35604T).

2023.11.24.

https://doi.org/10.1007/s00284-023-03523-6