International journal of Molecular Sciences

eISSN: 1422-0067

2022, 23, 10416

SCIE

[논문]Innate Immune Response Analysis in Meniscus Xenotransplantation Using Normal and Triple Knockout Jeju Native Pigs

Seungwon Yoon

윤승원, 손영옥, 허창기

Seungwon Yoon, Yunhui Min, Chungyu Park, Dahye Kim, Yunji Heo, Mangeun Kim, Eugene Son, Mrinmoy Ghosh, Young-Ok Son and Chang-Gi Hur

Although allogenic meniscus grafting can be immunologically safe, it causes immune re￾jection due to an imbalanced tissue supply between donor and recipient. Pigs are anatomically and
physiologically similar to adult humans and are, therefore, considered to be advantageous xeno￾transplantation models. However, immune rejection caused by genetic difference damages the do￾nor tissue and can sometimes cause sudden death. Immune rejection is caused by genes; porcine
GGTA1, CMAH, and B4GLANT2 are the most common. In this study, we evaluated immune cells
infiltrating the pig meniscus transplanted subcutaneously into BALB/c mice bred for three weeks.
We compared the biocompatibility of normal Jeju native black pig (JNP) meniscus with that of triple
knockout (TKO) JNP meniscus (α-gal epitope, N-glycolylneuraminic acid (Neu5Gc), and Sd (a)
epitope knockout using CRISPR-Cas 9). Mast cells, eosinophils, neutrophils, and macrophages were
found to have infiltrated the transplant boundary in the sham (without transplantation), normal
(normal JNP), and test (TKO JNP) samples after immunohistochemical analysis. When compared
to normal and sham groups, TKO was lower. Cytokine levels did not differ significantly between
normal and test groups . Because chronic rejection can occur after meniscus transplantation associ￾ated with immune cell infiltration, we propose studies with multiple genetic editing to prevent im￾mune rejection.

2022.09.08.

https://doi.org/10.3390/ijms231810416