이관욱

[논문] OsPPR19, a rice pentatricopeptide repeat protein, is essential for mitochondrial biogenesis and seed development

biologia plantarum

0.8

0006-3134

68

SCIE

Despite the accumulating evidence showing the essential role of pentatricopeptide repeat (PPR) proteins in organellar
biogenesis and plant development in Arabidopsis thaliana and maize (Zea mays), the functions of most PPR proteins
in rice (Oryza sativa) are still unknown. A former study demonstrated that the mitochondria-localized Arabidopsis
PPR19 is crucial for mitochondrial function and normal plant growth and development. In this study, we characterized
the functional role of a rice ortholog (LOC_Os12g04110) of Arabidopsis PPR19 protein. The loss-of-function osppr19
mutant displayed delayed seed germination and stunted root and seedling growth compared with wild-type. The height
of the osppr19 mutant was significantly shorter, and the grain mass of the mutant was lower than that of the wild-type.
The osppr19 mutant carried few filled grains and a higher number of aborted seeds than the wild-type. The structures
of mitochondria in the osppr19 mutant were abnormal, and more reactive oxygen species were accumulated in
the mutant, suggesting defective mitochondrial biogenesis and function in the osppr19 mutant. Importantly, the amount
of mature mitochondrial transcripts was significantly decreased in the mutant. Taken together, these results suggest that
the mitochondrial OsPPR19 is essential for mitochondrial biogenesis and function, which is crucial for plant growth
and development of rice grain.

Keywords: intron splicing, mitochondria, pentatricopeptide repeat, rice, seed development.

Kwanuk LEE*, Su Jung PARK, Yeon-Ok KIM, Jong-Seong JEON and Hunseung KANG*

2024.10.08.

DOI: 10.32615/bp.2024.014