김세재

[논문] Effects of p-coumaric acid on microRNA expression profiles in SNU-16 human gastric cancer cells

Genes & Genomics

1.188

2092-9293

42

SCIE

Background
p-Coumaric acid (p-CA), a phenolic compound abundantly found in edible plants, was found to induce apoptosis in SNU-16 gastric cancer cells. However, the efects of p-CA on the microRNA expression pattern in SNU-16 cells have not been reported.

Objectives
The primary objective of this study was to investigate microRNA expression profles in p-CA-treated SNU-16 cells.

Methods
SNU-16 cells were treated with 1.5 mM p-CA for 48 h. High-throughput RNA sequencing was performed. The accuracy and validity of the RNA sequencing results were tested by real-time PCR. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the diferentially expressed microRNAs (DEMs).

Results
Thirty-two DEMs were identifed between p-CA-treated and control cells, 9 of which were upregulated, and 23 downregulated. GO and KEGG pathway analyses of the target genes indicated that these genes were involved in a variety of biological functions and several cancer-related pathways. The miRNA interactive network analysis identifed hsa-miR 125a-5p, hsa-miR-30a-5p, and hsa-miR-7-5p as the major nodes.

Conclusions
Our fndings indicated that p-CA exerted its anticancer efects in SNU-16 gastric cancer cells by modulating the expression of certain microRNAs, providing a better understanding of the anticancer properties of p-CA in gastric cancer cells.

Keywords
MicroRNA · SNU-16 cell · p-Coumaric acid · Next-generation sequencing

Jang, Mi Gyeong; Ko, Hee Chul; Kim, Se-Jae;

2020.05.27(online)

https://doi.org/10.1007/s13258-020-00944-6