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[논문] Aqueous extract of freeze-dried Protaetia brevitarsis larvae promotes osteogenesis by activating β-catenin signaling

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논문제목(Title)

[논문] Aqueous extract of freeze-dried Protaetia brevitarsis larvae promotes osteogenesis by activating β-catenin signaling

학술지명(Journal)

Asian Pacific Journal of Tropical Biomedicine

ImpactFactor

1.514

ISSN_ISBN

2221-1691

학술지볼륨권호(Volume)

12(3)

SCI구분

SCIE

초록(Abstract)

Objective:
To investigate the effect of an aqueous extract of Protaetia brevitarsis (AEPB) on osteogenesis using preosteoblast MC3T3-E1 cells and zebrafish larvae.

Methods:
Flow cytometric analysis was used to measure the cytotoxicy. Alkaline phosphatase activity was detetmined using p-nitrophenyl phosphate as a substrate. Calcium deposition was detected using alizarin red staining along with osteogenic marker expression in preosteoblast MC3T3E1 cells. In addition, vertebral formation in zebrafish larvae was detected using calcein staining and osteogenic gene expression.

Results:
AEPB highly promoted the expression of osteogenic markers including runt-related transcription factor 2, osterix, and alkaline phosphatase, along with elevated levels of mineralization in MC3T3-E1 cells. Moreover, AEPB accelerated vertebral formation in zebrafish larvae accompanied by upregulated expression of osteogenic genes. FH535, an inhibitor of Wnt/β-catenin, suppressed AEPB-induced osteogenic gene expression and vertebral formation, indicating that AEPB stimulates osteogenesis by activating the Wnt/β-catenin signaling pathway.

Conclusions:
AEPB stimulates osteoblast differentiation and bone formation by activating β-catenin. Therefore, AEPB is a promising material that induces osteogenesis, and is useful for the treatment of bone resorption diseases.

저자명(Author)

Jayasingha Arachchige Chathuranga Chanaka Jayasingha, Kyoung Tae Lee, Yung Hyun Choi, Chang-Hee Kang, Mi-Hwa Lee, Gi-Young Kim

학술지출판일자(PublicationDate)

2022.05.10.

DMP

연구책임자 / 소속

김기영

당해연구기간

2022.03.01-2023.02.28

공개 및 라이선스

공개 일자

2023-01-07

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  • Version 1 2023-01-04
Cite as
Jayasingha Arachchige Chathuranga Chanaka Jayasingha, Kyoung Tae Lee, Yung Hyun Choi, Chang-Hee Kang, Mi-Hwa Lee, Gi-Young Kim, 2022.05.10., [논문] Aqueous extract of freeze-dried Protaetia brevitarsis larvae promotes osteogenesis by activating β-catenin signaling
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