김장훈

[논문] An investigation of the inhibitory mechanism of alpha-glucosidase by chysalodin from Aloe vera

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

4.082

0141-8130

147

SCIE

The aim of our study is to provide new type skeleton of α-glucosidase inhibitor from food source. A new 6′-O-(E)–cinnamoyl-7-methoxy-aloin A (1) was isolated from Aloe vera. Also, known chysalodin (2) was the first report for A. vera. These structures were identified with based on HR-ESI Mass and 1/2D-NMR spectra. These isolated compounds were tested for their inhibitory activities against α-glucosidase by using spectrophotometer.
Of these, compound 2 exhibited inhibitory activity, with an IC50 value of 13.4 ± 1.5 μM. An enzyme kinetic study identified the mechanism of binding of the ligand with the enzyme; the ligand binds in the active site of the enzyme in a competitive mode. Additionally, fluorescence quenching between the ligand and receptor revealed a two-to-one reaction. Finally, this finding provides that anthraquinone dimer (2) could be a starting point for the design of new class of α-glucosidase inhibitor.

Kim, Jang Hoon; Cho, Chong Woon; Lee, Jung In; Vinh, Le Ba; Kim, Kyung Tae; Cho, In Sook;

2020.01.08.

https://doi.org/10.1016/j.ijbiomac.2020.01.076