정만영

[논문] Kinetic, Genomic, and Physiological Analysis Reveals Diversity in the Ecological Adaptation and Metabolic Potential of Brachybacterium equifaecis sp. nov. Isolated from Horse Feces

Microbiology Spectrum

3.7

2165-0497

SCIE

Brachybacterium species have been identified in various ecological niches and belong to the family Dermabacteriaceae within the phylum Actinobacteria. In this study, we isolated a novel Brachybacterium equifaecis JHP9 strain from horse feces and compared its kinetic, biochemical, and genomic features with those of other Brachybacterium
strains. Moreover, comparative genomic analysis using publicly available Brachybacterium genomes was performed to determine the properties involved in their ecological adaptation and metabolic potential. Novel species delineation was
determined phylogenetically through 16S rRNA gene similarity (up to 97.9%), average nucleotide identity (79.5–82.5%), average amino acid identity (66.7–75.8%), and in silico DNA-DNA hybridization (23.7–27.9) using closely related strains. This study also presents the first report of the kinetic properties of Brachybacterium species. Most of
the Brachybacterium strains displayed high oxygen (Km(app) =1.6–24.2 µM) and glucose (Km(app) =0.73–1.22 µM) affinities, which may manifest niche adaptations. Various carbohydrate metabolisms under aerobic and anaerobic conditions, antibiotic resistance, mobile genetic elements, carbohydrate-active enzymes, lactic acid production, and
the clustered regularly interspaced short palindromic repeats-Cas and bacteriophage exclusion systems were observed in the genotypic and/or phenotypic properties of Brachybacterium species, suggesting their genome flexibility, defense mechanisms, and adaptability. Our study contributes to the knowledge of the kinetic, physiological, and
genomic properties of Brachybacterium species, including the novel JHP9 strain, which advocates for their tolerant and thriving nature in various environments, leading to their ecological adaptation.

Adeel Farooq, Myunglip Lee, Saem Han, Gi-Yong Jung, So-Jeong Kim, Man-Young Jung

2023.09.14.

DOI: 10.1128/spectrum.05048-22